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| 3. |  | SAVAREZ, T.; SILVA, L. O. G. da; SILVA, M. J.; SCALOPPI JR. E. J.; GONÇALVES, P. S.; MARTINS, M. A. Monitoramento das propriedades tecnológicas da borracha natural dos clones de seringueira RRIM 710, RRIM 728, RRIM 729 E RRIM 806 de janeiro a junho de 2019. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 466/470| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | SAVAREZ, T.; SILVA, L. O. G.; SILVA, M. J.; SCALOPPI JR, E. J.; GONÇALVES, P. S.; MARTINS, M. A. Caracterização das propriedades tecnológicas da borracha natural dos clones de seringueira RRIM 710, RRIM 728, RRIM 729 e RRIM 806. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. Editores técnicos: Alexandre Berndt, Ana Rita de Araujo Nogueira, Lea Chapaval, Marcelo Mattos Cavallari, Manuel Antonio Chagas Jacinto. 67 Embrapa Pecuária Sudeste. Documentos, 134.| Biblioteca(s): Embrapa Instrumentação. |
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| 5. | | SILVA, L. O. G.; SAVAREZ, T.; SILVA, M. J.; SCALOPPI JR, E. J.; GONÇALVES, P. S.; MARTINS, M. A. Avaliação das propriedades tecnológicas da borracha natural de novos clones de seringueira PC 96, PC 141 e IRCA 111. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. Editores técnicos: Alexandre Berndt, Ana Rita de Araujo Nogueira, Lea Chapaval, Marcelo Mattos Cavallari, Manuel Antonio Chagas Jacinto. 65 Embrapa Pecuária Sudeste. Documentos, 134.| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | ZENATTI, S.; SAVAREZ, T.; PARIS, E. C.; SCALOPPI JR, E. J.; GONÇALVES, P. S.; MARTINS, M. A. Efeito nas propriedades térmicas da concentração de titanato de bário em nanocompósitos com borracha natural. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 668 - 672| Biblioteca(s): Embrapa Instrumentação. |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
29/11/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NICKEL, O.; SILVA, F. N; FAJARDO, T. V. M.; GORAYEB, E. S. |
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Afiliação: |
OSMAR NICKEL, CNPUV; Fabio N. Silva, Programa de Pós-graduação em Produção Vegetal, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV; Eduardo E. Gorayeb, Programa de Pós-graduação em Produção Vegetal, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil. |
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Título: |
Characterization and genetic variability of coat protein genes of Apple chlorotic leaf spot virus isolates from southern Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 43, n. 2, p.109-116, 2018. |
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Páginas: |
8 |
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DOI: |
https://doi.org/10.1007/s40858-017-0197-6 |
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Idioma: |
Inglês |
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Notas: |
https://doi.org/10.1007/s40858-017-0197-6 |
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Conteúdo: |
Apple chlorotic leaf spot virus(ACLSV) infects temperate rosaceous fruit trees worldwide and causes a wide range of diseases that are economically highly damaging. This study was carried out to analyze genetic variability of regional ACLSV isolates and compare it with ACLSV isolates from other parts of the world. Nineteen amplicons of ACLSV, corresponding to the coat protein (CP) gene of isolates from apple, plum, and nectarine, from two states in southern Brazil, have been analyzed for genetic variation and compared phylogenetically among themselves and with other sequences available in GenBank. Sequences identities among complete CP genes of these isolates ranged from 87.5 to 100% and 92.2 to 100% at the nucleotide (nt) and the deduced amino acid (daa) levels, respectively. In the comparison with isolates from Asia, Europe and North America, identities were 68.4 to 100% and 72.5 to 100% at nt and daa levels, respectively. Phylogenetic trees based on nucleotide sequences showed that these isolates grouped into two clusters, cluster 1 containing apple isolates and cluster 2 comprising apple, plum and nec- tarine isolates. Most Brazilian isolates showed conserved signatures (Ser 40 ,Leu 59 ,Tyr 75 ,Thr 130 and Leu 184 )intheirCPs, which place them with type B6 isolates. However, some Brazilian isolates were found to be variants of type B6. These analyzes indicated that Brazilian isolates had lower genetic variability compared to isolates from China, India and Japan and that the CP genes were under negative selection. The greatest diversity of nucleotides was observed in the central portion of the CP gene, represented predominantly by synonymous substitutions. One natural recombinant was detected among ACLSV isolates from Brazil. Keywords ACLSV . Molecular characterization. Variability. Phylogeny. Recombination. Selection. MenosApple chlorotic leaf spot virus(ACLSV) infects temperate rosaceous fruit trees worldwide and causes a wide range of diseases that are economically highly damaging. This study was carried out to analyze genetic variability of regional ACLSV isolates and compare it with ACLSV isolates from other parts of the world. Nineteen amplicons of ACLSV, corresponding to the coat protein (CP) gene of isolates from apple, plum, and nectarine, from two states in southern Brazil, have been analyzed for genetic variation and compared phylogenetically among themselves and with other sequences available in GenBank. Sequences identities among complete CP genes of these isolates ranged from 87.5 to 100% and 92.2 to 100% at the nucleotide (nt) and the deduced amino acid (daa) levels, respectively. In the comparison with isolates from Asia, Europe and North America, identities were 68.4 to 100% and 72.5 to 100% at nt and daa levels, respectively. Phylogenetic trees based on nucleotide sequences showed that these isolates grouped into two clusters, cluster 1 containing apple isolates and cluster 2 comprising apple, plum and nec- tarine isolates. Most Brazilian isolates showed conserved signatures (Ser 40 ,Leu 59 ,Tyr 75 ,Thr 130 and Leu 184 )intheirCPs, which place them with type B6 isolates. However, some Brazilian isolates were found to be variants of type B6. These analyzes indicated that Brazilian isolates had lower genetic variability compared to isolates from China, India and Japan and that t... Mostrar Tudo |
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Palavras-Chave: |
ACLSV (Apple chlorotic leaf spot virus); Apple; Molecular characterization; Recombination; Selection; Temperate rosaceous fruit trees. |
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Thesaurus NAL: |
Genetic recombination; Phylogeny; Variability. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190140/1/Nickel2018-Article-CharacterizationAndGeneticVari.pdf
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Marc: |
LEADER 02828naa a2200301 a 4500
001 2080993
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008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-017-0197-6$2DOI
100 1 $aNICKEL, O.
245 $aCharacterization and genetic variability of coat protein genes of Apple chlorotic leaf spot virus isolates from southern Brazil.$h[electronic resource]
260 $c2018
300 $a8
500 $ahttps://doi.org/10.1007/s40858-017-0197-6
520 $aApple chlorotic leaf spot virus(ACLSV) infects temperate rosaceous fruit trees worldwide and causes a wide range of diseases that are economically highly damaging. This study was carried out to analyze genetic variability of regional ACLSV isolates and compare it with ACLSV isolates from other parts of the world. Nineteen amplicons of ACLSV, corresponding to the coat protein (CP) gene of isolates from apple, plum, and nectarine, from two states in southern Brazil, have been analyzed for genetic variation and compared phylogenetically among themselves and with other sequences available in GenBank. Sequences identities among complete CP genes of these isolates ranged from 87.5 to 100% and 92.2 to 100% at the nucleotide (nt) and the deduced amino acid (daa) levels, respectively. In the comparison with isolates from Asia, Europe and North America, identities were 68.4 to 100% and 72.5 to 100% at nt and daa levels, respectively. Phylogenetic trees based on nucleotide sequences showed that these isolates grouped into two clusters, cluster 1 containing apple isolates and cluster 2 comprising apple, plum and nec- tarine isolates. Most Brazilian isolates showed conserved signatures (Ser 40 ,Leu 59 ,Tyr 75 ,Thr 130 and Leu 184 )intheirCPs, which place them with type B6 isolates. However, some Brazilian isolates were found to be variants of type B6. These analyzes indicated that Brazilian isolates had lower genetic variability compared to isolates from China, India and Japan and that the CP genes were under negative selection. The greatest diversity of nucleotides was observed in the central portion of the CP gene, represented predominantly by synonymous substitutions. One natural recombinant was detected among ACLSV isolates from Brazil. Keywords ACLSV . Molecular characterization. Variability. Phylogeny. Recombination. Selection.
650 $aGenetic recombination
650 $aPhylogeny
650 $aVariability
653 $aACLSV (Apple chlorotic leaf spot virus)
653 $aApple
653 $aMolecular characterization
653 $aRecombination
653 $aSelection
653 $aTemperate rosaceous fruit trees
700 1 $aSILVA, F. N
700 1 $aFAJARDO, T. V. M.
700 1 $aGORAYEB, E. S.
773 $tTropical Plant Pathology$gv. 43, n. 2, p.109-116, 2018.
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Embrapa Uva e Vinho (CNPUV) |
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| 1. | | 0900875, RELATORIO TECNICO ANUAL. EMBRAPA. CNPSA, EMBRAPA-CNPSA, Concordia-SC
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